3-D Bioprinter Makes Beating Heart Tissue

January 24, 2017

News Release
January 24, 2017

EEG

Patients with heart failure who aren’t faring well on cardiac medication but also aren’t candidates for complex cardiac surgeries could one day have their hearts repaired with cardiac patches being created at Johns Hopkins Medicine.

Pediatric cardiac surgeon Narutoshi Hibino is using a 3-D bioprinter to create cardiac tissue that could one day surgically patch damaged hearts.

“This technology is much simpler compared to a heart transplant or ventricular assist device,” says Hibino. “It has the potential to improve patient quality of life.”

To treat damaged heart tissue, some researchers have been studying the efficiency of stem cell injections; others are developing cardiac patches that contain man-made elements, like engineered scaffolding upon which the tissue grows.

Hibino’s cardiac muscle does not contain scaffolding. The tissue is created by a 3-D printer that helps form stem cells into tiny balls and then inspects them for their uniformity. Then, much like a series of miniature shish kebabs, the printer skewers the cell balls on an array of needles. The printer’s Japanese manufacturer refers to this as the “kenzan method” because the printer’s collection of needles look similar to a kenzan, the spiky pad used to arrange plants in traditional Japanese flower arranging.

Those cells fuse together after a few days. The needles are then carefully pulled out, leaving beating heart tissue that can live for a month in a lab dish.

Hibino hopes to begin testing the tissue patches in rats next year.

The 3-D printer is not dedicated to a particular research core, but Hibino says it’s available for other Johns Hopkins Medicine research projects in Baltimore, like creating samples of organs that can then be used as three-dimensional testing material to study the efficiency of new drugs. Hibino can be reached at nhibino1@jhmi.edu.